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KMID : 0359320120520030169
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Korean Journal of Veterinary Research
2012 Volume.52 No. 3 p.169 ~ p.176
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Isolation, characterization, and evaluation of Bacillus thuringiensis isolated from cow milk
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Kweon Chang-Hee
Kang Hyun-Mi
Moon Jin-San
Kim Jong-man
Kang Seung-Won
Lee Hee-Soo
Jang Geum-Chag
Choi Sang-Yoon
Kwon Hyog-Young
Kim Eun-Hye
Pyo Suhk-Neung
Rhee Dong-Kwon
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Abstract
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Probiotics colonize the intestines and exert an antibacterial effect on pathogens. Therefore, probiotics could be used as a preventive agent against lethal infections. To isolate probiotic microorganisms, 116 bacterial strains were isolated from healthy cow’s milk and were subjected to Gram-stain, morphology and biochemical analyses, Vitek analysis, and 16S rRNA analysis. One of the strains identified as Bacillus (B.) thuringiensis 87 was found to grow very well at pH 4.0~7.0 and to be resistant to high concentrations of bile salts (0.3~0.9% w/v). B. thuringiensis was susceptible to the antibiotics used in the treatment of bovine mastitis, yet it exhibited an antimicrobial effect against Staphylococcus (S.) aureus 305. Moreover, it protected mice from experimental lethal infections of E. coli O55, Salmonella typhimurium 01D, and S. aureus 305 through a significant induction of interferon-γ, even at four-week post-administration oKeywords : Bacillus thuringiensis, interferon-γ, probiotics
f B. thuringiensis. Although oral administration of B. thuringiensis 87 did not provide significant protection against these lethal challenges, these results suggest that B. thuringiensis 87 could be a feasible candidate as a probiotic strain.
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KEYWORD
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Bacillus thuringiensis, interferon-γ, probiotics
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